Epifluorescence is an optical set-up for a fluorescence microscope in which the objective lens is used both to focus ultraviolet light on the specimen and collect fluorescent light from the specimen. Epifluorescence is more efficient than transmitted fluorescence, in which a separate lens or condenser is used to focus ultraviolet light on the specimen. Epifluorescence also allows fluorescence microscopy to be combined with another type on the same microscope.

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